Dataset Information

Novel Escherichia coli-Infecting Bacteriophages Isolated from Uganda That Target Human Clinical Isolates.

ABSTRACT:

Background

The antimicrobial resistance catastrophe is a growing global health threat and predicted to be worse in developing countries. Phages for Global Health (PGH) is training scientists in these regions to isolate relevant therapeutic phages for pathogenic bacteria within their locality, and thus contributing to making phage technology universally available.

Materials and methods

During the inaugural PGH workshop in East Africa, samples from Ugandan municipal sewage facilities were collected and two novel Escherichia coli lytic phages were isolated and characterized.

Results

The phages, UP19 (capsid diameter ∼100 nm, contractile tail ∼120/20 nm) and UP30 (capsid diameter ∼70 nm, noncontractile tail of ∼170/20 nm), lysed ∼82% and ∼36% of the 11 clinical isolates examined, respectively. The genomes of UP19 (171.402 kb, 282 CDS) and UP30 (49.834 kb, 75 CDS) closely match the genera Dhakavirus and Tunavirus, respectively.

Conclusion

The phages isolated have therapeutic potential for further development against E. coli infections.

SUBMITTER: Nale JY

PROVIDER: S-EPMC10574529 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature

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Novel <i>Escherichia coli</i>-Infecting Bacteriophages Isolated from Uganda That Target Human Clinical Isolates.

Nale Janet Yakubu JY Chan Benjamin B Nnadi Nnaemeka Emmanuel NE Cheng Jeffrey Kwok Jone JKJ Matts Susan S Nezam-Abadi Neda N Turkington Christopher Jason Richard CJR Charreton Lucie Manon LM Bola Harroop H Nazir Ramez R Hoza Abubakar Shaaban AS Wamala Samuel Posian SP Ibanda Ivan I Maina Alice Nyambura AN Apopo Auleria Ajiambo AA Msoffe Venance Theophil VT Moremi Nyambura N Moore Grace Wanjiru GW Asiimwe Ismail I Namatovu Alice A Mutumba Paul P Kamya Deus D Nabunje Ritah R Nakabugo Immaculate I Kazwala Rudovick Ruben RR Kangethe Erastus E Negash Abel Abera AA Watelo Arthur Kalyebi AK Bukamba Nelson N Muhindo Gideon G Lubowa Nathan Musisi NM Jillani Ngalla N Nyachieo Atunga A Nasinyama George G Nakavuma Jesca J Millard Andrew A Nagel Tobi Elaine TE Clokie Martha Rebecca Jane MRJ

PHAGE (New Rochelle, N.Y.) 20230920 3

<h4>Background</h4>The antimicrobial resistance catastrophe is a growing global health threat and predicted to be worse in developing countries. Phages for Global Health (PGH) is training scientists in these regions to isolate relevant therapeutic phages for pathogenic bacteria within their locality, and thus contributing to making phage technology universally available.<h4>Materials and methods</h4>During the inaugural PGH workshop in East Africa, samples from Ugandan municipal sewage facilitie ...[more]

PMID: 37841386

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Project description:IntroductionUropathogenic E. coli is the leading cause of Urinary tract infections (UTIs), contributing to 80-90% of all community-acquired and 30-50% of all hospital-acquired UTIs. Biofilm forming Uropathogenic E. coli are associated with persistent and chronic inflammation leading to complicated and or recurrent UTIs. Biofilms provide an environment for poor antibiotic penetration and horizontal transfer of virulence genes which favors the development of Multidrug-resistant organisms (MDRO). Understanding biofilm formation and antimicrobial resistance determinants of Uropathogenic E. coli strains will provide insight into the development of treatment options for biofilm-associated UTIs. The aim of this study was to determine the biofilm forming capability, presence of virulence genes and antimicrobial susceptibility pattern of Uropathogenic E. coli isolates in Uganda.MethodsThis was a cross-sectional study carried in the Clinical Microbiology and Molecular biology laboratories at the Department of Medical Microbiology, Makerere University College of Health Sciences. We randomly selected 200 Uropathogenic E. coli clinical isolates among the stored isolates collected between January 2018 and December 2018 that had significant bacteriuria (> 105 CFU). All isolates were subjected to biofilm detection using the Congo Red Agar method and Antimicrobial susceptibility testing was performed using the Kirby disk diffusion method. The isolates were later subjected PCR for the detection of Urovirulence genes namely; Pap, Fim, Sfa, Afa, Hly and Cnf, using commercially designed primers.ResultsIn this study, 62.5% (125/200) were positive biofilm formers and 78% (156/200) of these were multi-drug resistant (MDR). The isolates were most resistant to Trimethoprim sulphamethoxazole and Amoxicillin (93%) followed by gentamycin (87%) and the least was imipenem (0.5%). Fim was the most prevalent Urovirulence gene (53.5%) followed by Pap (21%), Sfa (13%), Afa (8%), Cnf (5.5%) and Hyl (0%).ConclusionsWe demonstrate a high prevalence of biofilm-forming Uropathogenic E. coli strains that are highly associated with the MDR phenotype. We recommend routine surveillance of antimicrobial resistance and biofilm formation to understand the antibiotics suitable in the management of biofilm-associated UTIs.