Dataset Information

Aspirin inhibits rotavirus replication and alters rat gut microbial composition.

ABSTRACT:

Background

Aspirin is widely used to treat various clinical symptoms. Evidence suggests that aspirin has antiviral properties, but little is known about its specific effect against rotavirus.

Methods

MA104, Caco-2, and CV-1 cells were infected with rotavirus, and aspirin was added after 12 h. Viral mRNA and titer levels were measured by qRT-PCR and immunofluorescence assays. For in vivo validation, forty specific-pathogen-free SD rats were randomly divided into oral aspirin (ASP) groups and control (NC) groups. 16 S rRNA gene sequencing was performed to identify gut microbiota. After 6 months of continuous ASP/NC administration, the rats were infected with rotavirus. Fecal samples were collected over a 30-day time course, and viral levels were quantified. Proinflammatory cytokines/chemokine levels were measured by ELISA.

Results

Aspirin inhibited rotavirus infection in cell lines and in rats. The effects of aspirin on viral replication were associated with the alteration of gut microbiota composition by aspirin, including increased abundance of Firmicutes and decreased abundance of Bacteroidetes after aspirin treatment. Mechanistically, aspirin reduced IL-2 and IL-10 levels, and increased IRF-1 and COX-2 levels. Aspirin blocked rotavirus replication in vitro and in vivo, which might be related to effects on IRF-1, COX-2, chemokines, and gut microbial composition.

Conclusion

These results indicate that long-term oral aspirin administration reduces rotavirus infection. Intestinal virus infection may be suppressed in elderly patients who take aspirin for a long time. The change of their Gut microbiota may lead to functional disorder of the intestinal tract, which may provide some reference for clinical adjuvant probiotics treatment.

SUBMITTER: Zhao W

PROVIDER: S-EPMC10580602 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature

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Publications

Aspirin inhibits rotavirus replication and alters rat gut microbial composition.

Zhao Wei W Li ZhouPing Z Yu Mei Ling ML Liu Yang Y Liu Chang Cheng CC Jia Xue Jiao XJ Liu Meng Qi MQ Li Yong Gang YG

Virology journal 20231017 1

<h4>Background</h4>Aspirin is widely used to treat various clinical symptoms. Evidence suggests that aspirin has antiviral properties, but little is known about its specific effect against rotavirus.<h4>Methods</h4>MA104, Caco-2, and CV-1 cells were infected with rotavirus, and aspirin was added after 12 h. Viral mRNA and titer levels were measured by qRT-PCR and immunofluorescence assays. For in vivo validation, forty specific-pathogen-free SD rats were randomly divided into oral aspirin (ASP) ...[more]

PMID: 37848986

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Dataset Information

Aspirin inhibits rotavirus replication and alters rat gut microbial composition.

Background

Methods

Results

Conclusion

Publications

Aspirin inhibits rotavirus replication and alters rat gut microbial composition.

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