Dataset Information

In Vitro Evaluation of Probiotic Properties of Two Novel Probiotic Mixtures, Consti-Biome and Sensi-Biome.

ABSTRACT: Changes in the gut microbiome cause recolonization by pathogens and inflammatory responses, leading to the development of intestinal disorders. Probiotics administration has been proposed for many years to reverse the intestinal dysbiosis and to enhance intestinal health. This study aimed to evaluate the inhibitory effects of two newly designed probiotic mixtures, Consti-Biome and Sensi-Biome, on two enteric pathogens Staphylococcus aureus and Escherichia coli that may cause intestinal disorders. Additionally, the study was designed to evaluate whether Consti-Biome and Sensi-Biome could modulate the immune response, produce short-chain fatty acids (SCFAs), and reduce gas production. Consti-Biome and Sensi-Biome showed superior adhesion ratios to HT-29 cells and competitively suppressed pathogen adhesion. Moreover, the probiotic mixtures decreased the levels of pro-inflammatory cytokines, such as tumor necrosis factor-α, interleukin (IL)-6 and IL-1β. Cell-free supernatants (CFSs) were used to investigate the inhibitory effects of metabolites on growth and biofilms of pathogens. Consti-Biome and Sensi-Biome CFSs exhibited antimicrobial and anti-biofilm activity, where microscopic analysis confirmed an increase in the number of dead cells and the structural disruption of pathogens. Gas chromatographic analysis of the CFSs revealed their ability to produce SCFAs, including acetic, propionic, and butyric acid. SCFA secretion by probiotics may demonstrate their potential activities against pathogens and gut inflammation. In terms of intestinal symptoms regarding abdominal bloating and discomfort, Consti-Biome and Sensi-Biome also inhibited gas production. Thus, these two probiotic mixtures have great potential to be developed as dietary supplements to alleviate the intestinal disorders.

SUBMITTER: Jang YJ

PROVIDER: S-EPMC10580887 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature

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In Vitro Evaluation of Probiotic Properties of Two Novel Probiotic Mixtures, Consti-Biome and Sensi-Biome.

Jang You Jin YJ Min Bonggyu B Lim Jong Hyun JH Kim Byung-Yong BY

Journal of microbiology and biotechnology 20230619 9

Changes in the gut microbiome cause recolonization by pathogens and inflammatory responses, leading to the development of intestinal disorders. Probiotics administration has been proposed for many years to reverse the intestinal dysbiosis and to enhance intestinal health. This study aimed to evaluate the inhibitory effects of two newly designed probiotic mixtures, Consti-Biome and Sensi-Biome, on two enteric pathogens <i>Staphylococcus aureus</i> and <i>Escherichia coli</i> that may cause intest ...[more]

PMID: 37386724

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Project description:Current production of virgin plastics stands at approximately 0.4 billion tonnes annually, with significant applications in packaging, construction, and the automotive industry. Despite the utility of plastics, their waste management remains a challenge. This study focuses on upcycling plastic waste (PW) through mechanical recycling, which adds value to discarded plastics and aligns with sustainable practices. We have investigated the mechanical and thermal properties of ternary and quaternary blends of the most common polyolefin polymers, low-density polyethylene and polypropylene (PP), simulating typical PW mixtures. The blends were prepared using twin screw extrusion for melting, mixing, and strand extrusion. The strands from the extruder were guided through a water bath, optionally dried, and pelletized, to determine the processing conditions variations. Aliquots of the blends were directly taken from the extruder to form specimens from the melts. Additionally, the produced pellets were remolten to form specimens, resulting in a second heating cycle (HC). Thermal stability, calorimetric properties, surface morphology, and mechanical characteristics of the blends were analyzed. Results indicate that ternary blends exhibit higher thermal stability but lower mechanical strength compared with quaternary blends. The addition of PP slightly reduced the onset temperature in quaternary blends. The melting temperatures of the blends show minimal change with additional HCs or drying, suggesting the maintenance of thermal properties. The ternary blends exhibit consistent mechanical properties regardless of the processing conditions, but the breakup force and tensile modulus of the quaternary blends were lower with additional HCs. The surface morphological studies revealed increased cavitation with two HCs and improved surface smoothness with drying. These findings support the potential of mechanical recycling to produce marketable plastic grades from mixed PW.

Dataset Information

In Vitro Evaluation of Probiotic Properties of Two Novel Probiotic Mixtures, Consti-Biome and Sensi-Biome.

Publications

In Vitro Evaluation of Probiotic Properties of Two Novel Probiotic Mixtures, Consti-Biome and Sensi-Biome.

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