Streamlined structure determination by cryo-electron tomography and subtomogram averaging using TomoBEAR.

ABSTRACT: Structures of macromolecules in their native state provide unique unambiguous insights into their functions. Cryo-electron tomography combined with subtomogram averaging demonstrated the power to solve such structures in situ at resolutions in the range of 3 Angstrom for some macromolecules. In order to be applicable to the structural determination of the majority of macromolecules observable in cells in limited amounts, processing of tomographic data has to be performed in a high-throughput manner. Here we present TomoBEAR-a modular configurable workflow engine for streamlined processing of cryo-electron tomographic data for subtomogram averaging. TomoBEAR combines commonly used cryo-EM packages with reasonable presets to provide a transparent ("white box") approach for data management an

SUBMITTER: Balyschew N 

PROVIDER: S-EPMC10582028 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature