Histology and transcriptome insights into the early processes of intestinal anastomotic healing: a rat model.

ABSTRACT:

Background

Understanding the early processes underlying intestinal anastomotic healing is crucial to comprehend the pathophysiology of anastomotic leakage. The aim of this study was to assess normal intestinal anastomotic healing and disturbed healing in rats to investigate morphological, cellular and intrinsic molecular changes in the anastomotic tissue.

Method

Anastomoses were created in two groups of Wistar rats, using four sutures or 12 sutures to mimic anastomotic leakage and anastomotic healing respectively. At 6, 12, 24 hours and 2, 3, 5 and 7 days, anastomotic tissue was assessed macroscopically using the anastomotic complication score and histologically using the modified Ehrlich-Hunt score. Transcriptome analysis was performed to assess differences between anastomo

SUBMITTER: van Helsdingen CPM 

PROVIDER: S-EPMC10586197 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature