Functional characterization of alkaline phosphatases involved alarm pheromone in the vetch aphid Megoura viciae.

ABSTRACT: The alkaline phosphatases (ALPs) are highly promiscuous enzymes and have been extensively investigated in mammals for their medical significance, but their functional promiscuity is relatively poorly understood in insects. Here, we first identified four ALP genes (designated as MvALP1-4) in the vetch aphid Megoura viciae that contained one alkaline phosphatase site, three metal-binding sites, and varied other functional sites. Phylogenetic analysis, molecular docking and the spatiotemporal expression profiling of MvALP1-4 were very different, indicating a promiscuous functionality. We also found that MvALP4 involved the biosynthesis of aphid alarm pheromones (EβF) in vitro and in vivo. Finally, transcriptome analysis in the stimulated and unstimulated aph

SUBMITTER: Song X 

PROVIDER: S-EPMC10590853 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature