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A Cascade Signal Amplification Strategy for the Ultrasensitive Fluorescence Detection of Cu²⁺ via λ-Exonuclease-Assisted Target Recycling with Mismatched Catalytic Hairpin Assembly.

ABSTRACT: Herein, an ultrasensitive DNAzyme-based fluorescence biosensor for detecting Cu²⁺ was designed using the cascade signal amplification strategy, coupling λ-exonuclease-assisted target recycling and mismatched catalytic hairpin assembly (MCHA). In the designed detection system, the target, Cu²⁺, can activate the Cu²⁺-dependent DNAzyme to cause a cleavage reaction, releasing ssDNA (tDNA). Then, tDNA binds to hairpin DNA (H0) with an overhanging 5'-phosphorylated terminus to form dsDNA with a blunt 5'-phosphorylated terminus, which activates the dsDNA to be digested by λ-Exo and releases tDNA along with another ssDNA (iDNA). Subsequently, the iDNA initiates MCHA, which can restore the fluorescence of carboxyfluorescein (FAM) p

SUBMITTER: Liu Z

PROVIDER: S-EPMC10605925 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature

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Dataset Information

A Cascade Signal Amplification Strategy for the Ultrasensitive Fluorescence Detection of Cu²⁺ via λ-Exonuclease-Assisted Target Recycling with Mismatched Catalytic Hairpin Assembly.

OmicsDI is part of the ELIXIR infrastructure

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Dataset Information

A Cascade Signal Amplification Strategy for the Ultrasensitive Fluorescence Detection of Cu2+ via λ-Exonuclease-Assisted Target Recycling with Mismatched Catalytic Hairpin Assembly.

OmicsDI is part of the ELIXIR infrastructure

Tweets

A Cascade Signal Amplification Strategy for the Ultrasensitive Fluorescence Detection of Cu²⁺ via λ-Exonuclease-Assisted Target Recycling with Mismatched Catalytic Hairpin Assembly.