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Is the Neuropeptide PEN a Ligand of GPR83?

ABSTRACT: G protein-coupled receptor 83 (GPR83) is a class A G protein-coupled receptor with predominant expression in the cerebellum and proposed function in the regulation of food intake and in anxiety-like behavior. The neuropeptide PEN has been suggested as a specific GPR83 ligand. However, conflicting reports exist about whether PEN is indeed able to bind and activate GPR83. This study was initiated to evaluate PEN as a potential ligand of GPR83. Employing several second messenger and other GPCR activation assays as well as a radioligand binding assay, and using multiple GPR83 plasmids and PEN peptides from different sources, no experimental evidence was found to support a role of PEN as a GPR83 ligand.

SUBMITTER: Giesecke Y

PROVIDER: S-EPMC10606834 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature

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Is the Neuropeptide PEN a Ligand of GPR83?

Giesecke Yvonne Y Asimi Vahid V Stulberg Valentina V Kleinau Gunnar G Scheerer Patrick P Koksch Beate B Grötzinger Carsten C

International journal of molecular sciences 20231012 20

G protein-coupled receptor 83 (GPR83) is a class A G protein-coupled receptor with predominant expression in the cerebellum and proposed function in the regulation of food intake and in anxiety-like behavior. The neuropeptide PEN has been suggested as a specific GPR83 ligand. However, conflicting reports exist about whether PEN is indeed able to bind and activate GPR83. This study was initiated to evaluate PEN as a potential ligand of GPR83. Employing several second messenger and other GPCR acti ...[more]

PMID: 37894796

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