Metabolic Remodeling during Early Cardiac Lineage Specification of Pluripotent Stem Cells.

ABSTRACT: Growing evidence indicates that metabolites and energy metabolism play an active rather than consequential role in regulating cellular fate. Cardiac development requires dramatic metabolic remodeling from relying primarily on glycolysis in pluripotent stem cells (PSCs) to oxidizing a wide array of energy substrates to match the high bioenergetic demands of continuous contraction in the developed heart. However, a detailed analysis of how remodeling of energy metabolism contributes to human cardiac development is lacking. Using dynamic multiple reaction monitoring metabolomics of central carbon metabolism, we evaluated temporal changes in energy metabolism during human PSC 3D cardiac lineage specification. Significant metabolic remodeling occurs during the complete differentiation, yet temp

SUBMITTER: Bobori SN 

PROVIDER: S-EPMC10608731 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature