Immunized mice naturally process in silico-derived peptides from the nucleocapsid of SARS-CoV-2.

ABSTRACT:

Background

The nucleocapsid (N) protein of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is an excellent immunogen that promotes the production of high-titer antibodies. N protein-derived peptides identified using a bioinformatics approach can potentially be used to develop a new generation of vaccines or diagnostic methods for detecting SARS-CoV-2 and its variants. However, further studies must demonstrate their capacity to be naturally processed by the immune system.

Objective

We aimed to examine the in vivo processing and recognition of in silico-identified peptides using the serum of immunized animals with the complete protein.

Methods

Recombinant N (Nrec) protein was subcutaneously administered to six Balb/c mice. Enzyme-linked immunosorbent assay

SUBMITTER: Campos-Ruiz MA 

PROVIDER: S-EPMC10612231 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature