Single-cell RNAseq identifies clonally expanded antigen-specific T-cells following intradermal injection of gold nanoparticles loaded with diabetes autoantigen in humans.

ABSTRACT: Gold nanoparticles (GNPs) have been used in the development of novel therapies as a way of delivery of both stimulatory and tolerogenic peptide cargoes. Here we report that intradermal injection of GNPs loaded with the proinsulin peptide C19-A3, in patients with type 1 diabetes, results in recruitment and retention of immune cells in the skin. These include large numbers of clonally expanded T-cells sharing the same paired T-cell receptors (TCRs) with activated phenotypes, half of which, when the TCRs were re-expressed in a cell-based system, were confirmed to be specific for either GNP or proinsulin. All the identified gold-specific clones were CD8⁺, whilst proinsulin-specific clones were both CD8⁺ and CD4⁺. Proinsulin-specific CD8⁺ clones had a

SUBMITTER: Hanna SJ 

PROVIDER: S-EPMC10613693 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature