Ciliary localization of a light-activated neuronal GPCR shapes behavior.

ABSTRACT: Many neurons in the central nervous system produce a single primary cilium that serves as a specialized signaling organelle. Several neuromodulatory G-protein-coupled receptors (GPCRs) localize to primary cilia in neurons, although it is not understood how GPCR signaling from the cilium impacts circuit function and behavior. We find that the vertebrate ancient long opsin A (VALopA), a G_i-coupled GPCR extraretinal opsin, targets to cilia of zebrafish spinal neurons. In the developing 1-d-old zebrafish, brief light activation of VALopA in neurons of the central pattern generator circuit for locomotion leads to sustained inhibition of coiling, the earliest form of locomotion. We find that a related extraretinal opsin, VALopB, is also G_i-coupled, but is not targeted to ci

SUBMITTER: Winans AM 

PROVIDER: S-EPMC10614621 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature