Mating-induced ecdysone in the testis disrupts soma-germline contacts and stem cell cytokinesis.

ABSTRACT: Germline maintenance relies on adult stem cells to continually replenish lost gametes over a lifetime and respond to external cues altering the demands on the tissue. Mating worsens germline homeostasis over time, yet a negative impact on stem cell behavior has not been explored. Using extended live imaging of the Drosophila testis stem cell niche, we find that short periods of mating in young males disrupts cytokinesis in germline stem cells (GSCs). This defect leads to failure of abscission, preventing release of differentiating cells from the niche. We find that GSC abscission failure is caused by increased ecdysone hormone signaling induced upon mating, which leads to disrupted somatic encystment of the germline. Abscission failure is rescued by isolating males from females but

SUBMITTER: Roach TV 

PROVIDER: S-EPMC10614927 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature