Copper nanoparticles embedded fungal chitosan as a rational and sustainable bionanozyme with robust laccase activity for catalytic oxidation of phenolic pollutants.

ABSTRACT: Despite their potential for oxidation of persistent environmental pollutants, the development of rational and sustainable laccase nanozymes with efficient catalytic performance remains a challenge. Herein, fungal-produced chitosan-copper (CsCu) is proposed as a rational and sustainable bionanozyme with intrinsic laccase activity. The CsCu nanozyme was prepared by in situ reduction of copper on chitosan extracted from Irpex sp. isolate AWK2 a native fungus, from traditional fermented foods, yielding a low molecular weight chitosan with a 70% degree of deacetylation. Characterizations of the nanozyme using SEM-EDX, XRD, and XPS confirmed the presence of a multi-oxidation state copper on the chitosan matrix which is consistent with the composition of natural laccase. The laccase memetic activity was investigated using 2,4-DP as a substrate which oxidized to form a reddish-pink color with 4-AP (λ_max = 510 nm). The CsCu nanozyme showed 38% higher laccase activity than the pristine Cu NPs at pH 9, indicating enhanced activity in the presence of chitosan structure. Further, CsCu showed significant stability in harsh conditions and exhibited a lower K_m (0.26 mM) which is competitive with that reported for natural laccase. Notably, the nanozyme converted 92% of different phenolic substrates in 5 h, signifying a robust performance for environmental remediation purposes.

SUBMITTER: Mekonnen EG 

PROVIDER: S-EPMC10619478 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature