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AspFlex: Molecular Tools to Study Gene Expression and Regulation in Acinetobacter baumannii.

ABSTRACT: Acinetobacter baumannii is a Gram-negative nosocomial opportunistic pathogen frequently found in hospital settings, causing high incidence of in-hospital infections. It belongs to the ESKAPE group of pathogens (the "A" stands for A. baumannii), which are known to easily develop antibiotic resistances. It is crucial to create a molecular toolkit to investigate its basic biology, such as gene regulation. Despite A. baumannii having been a threat for almost two decades, an efficient and high-throughput plasmid system that can replicate in A. baumannii has not yet been developed. This study adapts an existing toolkit for Escherichia coli to meet A. baumannii's unique requirements and expands it by constructing a plasmid-based CRISPR interference (CRISPRi) system to generate gene knockdowns in A. baumannii.

SUBMITTER: Brychcy M

PROVIDER: S-EPMC10621034 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature

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AspFlex: Molecular Tools to Study Gene Expression and Regulation in Acinetobacter baumannii.

Brychcy Merlin M Kokodynski Alexis A Lloyd Devin D Godoy Veronica G VG

ACS synthetic biology 20230816 9

Acinetobacter baumannii is a Gram-negative nosocomial opportunistic pathogen frequently found in hospital settings, causing high incidence of in-hospital infections. It belongs to the ESKAPE group of pathogens (the "A" stands for A. baumannii), which are known to easily develop antibiotic resistances. It is crucial to create a molecular toolkit to investigate its basic biology, such as gene regulation. Despite A. baumannii having been a threat for almost two decades, an effi ...[more]

PMID: 37587063

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