NANOG expression in parthenogenetic porcine blastocysts is required for intact lineage specification and pluripotency.

ABSTRACT:

Objective

Nanog homeobox (NANOG) is a core transcription factor that contributes to pluripotency along with octamer binding transcription factor-4 (OCT4) and sex determining region-Y box-2 (SOX2). It is an epiblast lineage marker in mammalian pre-implantation embryos and exhibits a species-specific expression pattern. Therefore, it is important to understand the lineage of NANOG, the trophectoderm, and the primitive endoderm in the pig embryo.

Methods

A loss- and gain-of-function analysis was done to determine the role of NANOG in lineage specification in parthenogenetic porcine blastocysts. We analyzed the relationship between NANOG and pluripotent core transcription factors and other lineage makers.

Results

In NANOG-null late blastocysts, OCT4-, SOX2-, and SOX17-pos

SUBMITTER: Lee M 

PROVIDER: S-EPMC10623019 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature