Pan-cortical 2-photon mesoscopic imaging and neurobehavioral alignment in awake, behaving mice.

ABSTRACT: The flow of neural activity across the neocortex during active sensory discrimination is constrained by task-specific cognitive demands, movements, and internal states. During behavior, the brain appears to sample from a broad repertoire of activation motifs. Understanding how these patterns of local and global activity are selected in relation to both spontaneous and task-dependent behavior requires in-depth study of densely sampled activity at single neuron resolution across large regions of cortex. In a significant advance toward this goal, we developed procedures to record mesoscale 2-photon Ca²⁺ imaging data from two novel in vivo preparations that, between them, allow simultaneous access to nearly all of the mouse dorsal and lateral neocortex. As a proof of principl

SUBMITTER: Vickers ED 

PROVIDER: S-EPMC10634705 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature