RACK1 promotes Shigella flexneri actin-mediated invasion, motility, and cell-to-cell spreading.

ABSTRACT: Shigella flexneri is an intracellular bacterium that hijacks the host actin cytoskeleton to invade and disseminate within the colonic epithelium. Shigella's virulence factors induce actin polymerization, leading to bacterial uptake, actin tail formation, actin-mediated motility, and cell-to-cell spreading. Many host factors involved in the Shigella-prompted actin rearrangements remain elusive. Here, we studied the role of a host protein receptor for activated C kinase 1 (RACK1) in actin cytoskeleton dynamics and Shigella infection. We used time-lapse imaging to demonstrate that RACK1 facilitates Shigella-induced actin cytoskeleton remodeling at multiple levels during infection of epithelial cells. Silencing RACK1 expression impaired Shigella-induce

SUBMITTER: Valenzuela-Valderas KN 

PROVIDER: S-EPMC10637933 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature