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Tau forms synaptic nano-biomolecular condensates controlling the dynamic clustering of recycling synaptic vesicles.


ABSTRACT: Neuronal communication relies on the release of neurotransmitters from various populations of synaptic vesicles. Despite displaying vastly different release probabilities and mobilities, the reserve and recycling pool of vesicles co-exist within a single cluster suggesting that small synaptic biomolecular condensates could regulate their nanoscale distribution. Here, we performed a large-scale activity-dependent phosphoproteome analysis of hippocampal neurons in vitro and identified Tau as a highly phosphorylated and disordered candidate protein. Single-molecule super-resolution microscopy revealed that Tau undergoes liquid-liquid phase separation to generate presynaptic nanoclusters whose density and number are regulated by activity. This activity-dependent diffusion process allows Tau to translocate into the presynapse where it forms biomolecular condensates, to selectively control the mobility of recycling vesicles. Tau, therefore, forms presynaptic nano-biomolecular condensates that regulate the nanoscale organization of synaptic vesicles in an activity-dependent manner.

SUBMITTER: Longfield SF 

PROVIDER: S-EPMC10638352 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature

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Tau forms synaptic nano-biomolecular condensates controlling the dynamic clustering of recycling synaptic vesicles.

Longfield Shanley F SF   Mollazade Mahdie M   Wallis Tristan P TP   Gormal Rachel S RS   Joensuu Merja M   Wark Jesse R JR   van Waardenberg Ashley J AJ   Small Christopher C   Graham Mark E ME   Meunier Frédéric A FA   Martínez-Mármol Ramón R  

Nature communications 20231110 1


Neuronal communication relies on the release of neurotransmitters from various populations of synaptic vesicles. Despite displaying vastly different release probabilities and mobilities, the reserve and recycling pool of vesicles co-exist within a single cluster suggesting that small synaptic biomolecular condensates could regulate their nanoscale distribution. Here, we performed a large-scale activity-dependent phosphoproteome analysis of hippocampal neurons in vitro and identified Tau as a hig  ...[more]

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