Rapid diagnosis of Mycobacterium marinum infection using targeted nanopore sequencing: a case report.

ABSTRACT: Mycobacterium marinum (M. marinum) is a non-tuberculous mycobacterium (NTM) that can cause infectious diseases in aquatic animals and humans. Culture-based pathogen detection is the gold standard for diagnosing NTM infection. However, this method is time-consuming and has low positivity rates for fastidious organisms. Oxford Nanopore MinION sequencing is an emerging third-generation sequencing technology that can sequence DNA or RNA directly in a culture-independent manner and offers rapid microbial identification. Further benefits include low cost, short turnaround time, long read lengths, and small equipment size. Nanopore sequencing plays a crucial role in assessing drug resistance, clinical identification of microbes, and monitoring infectious diseases. Some report

SUBMITTER: Huang YY 

PROVIDER: S-EPMC10642934 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature