Dataset Information

OsCIPK9 Interacts with OsSOS3 and Affects Salt-Related Transport to Improve Salt Tolerance.

ABSTRACT: Salt is harmful to crop production. Therefore, it is important to understand the mechanism of salt tolerance in rice. CIPK genes have various functions, including regulating salt tolerance and other types of stress and nitrogen use efficiency. In rice, OsCIPK24 is known to regulate salt tolerance, but other OsCIPKs could also function in salt tolerance. In this study, we identified another OsCIPK-OsCIPK9-that can regulate salt tolerance. Knockout of OsCIPK9 in rice could improve salt tolerance. Through expression analyses, OsCIPK9 was found to be mainly expressed in the roots and less expressed in mature leaves. Meanwhile, OsCIPK9 had the highest expression 6 h after salt treatment. In addition, we proved the interaction between OsCIPK9 and OsSOS3. The RNA-seq data showed that OsCIPK9 strongly responded to salt treatment, and the transporters related to salt tolerance may be downstream genes of OsCIPK9. Finally, haplotype analyses revealed that Hap6 and Hap8 mainly exist in indica, potentially providing a higher salt tolerance. Overall, a negative regulator of salt tolerance, OsCIPK9, which interacted with OsSOS3 similarly to OsCIPK24 and influenced salt-related transporters, was identified, and editing OsCIPK9 potentially could be helpful for breeding salt-tolerant rice.

SUBMITTER: Zhou Z

PROVIDER: S-EPMC10647249 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature

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OsCIPK9 Interacts with OsSOS3 and Affects Salt-Related Transport to Improve Salt Tolerance.

Zhou Zhenling Z Tang Weijie W Sun Zhiguang Z Li Jingfang J Yang Bo B Liu Yan Y Wang Baoxiang B Xu Dayong D Yang Jianchang J Zhang Yunhui Y

Plants (Basel, Switzerland) 20231030 21

Salt is harmful to crop production. Therefore, it is important to understand the mechanism of salt tolerance in rice. <i>CIPK</i> genes have various functions, including regulating salt tolerance and other types of stress and nitrogen use efficiency. In rice, OsCIPK24 is known to regulate salt tolerance, but other OsCIPKs could also function in salt tolerance. In this study, we identified another OsCIPK-OsCIPK9-that can regulate salt tolerance. Knockout of <i>OsCIPK9</i> in rice could improve sa ...[more]

PMID: 37960079

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Project description:Salt stress limits plant growth and agricultural productivity and plants have evolved suitable mechanisms to adapt to salinity environments. It is important to characterize genes involved in plant salt tolerance, which will advance our understanding of mechanisms mediating salt tolerance and help researchers design ways to improve crop performances under high salinity environments. Here, we reported a CYP450 family member, CYP75B4, improves salt tolerance of rice seedlings by inducing flavonoid tricin and cell wall lignin accumulation. The CYP75B4 is highly expressed in tissues rich in cell walls and induced by salt treatment. Subcellular localization analysis revealed that CYP75B4 is localized in the endoplasmic reticulum (ER). The CYP75B4 overexpressing (CYP75B4-OE) lines showed significant enhancement in stem mechanical strength, whereas the cyp75b4 null mutants displayed weaker stems, as compared to the wild-type. Notably, the cyp75b4 and CYP75B4-OE lines showed decreased and improved, respectively, salt tolerance performances in terms of survival rate, ROS accumulation, and Na+/ K+ homeostasis. Additionally, the cyp75b4 mutants had a decreased tricin level, whereas CYP75B4-OE lines showed an increased tricin content, under both control or salinity conditions. Furthermore, treating the cyp75b4 mutants with tricin partly resorted salt stress tolerance to the wild-type levels, indicating a role of CYP75B4-mediated tricin production in rice response to salinity. Consistently, another tricin-deficient mutant cyp93g1 also displayed salt sensitivity and the tricin application could partly restore its salt-sensitive phenotypes. Moreover, the CYP75B4 significantly promotes lignin deposition in cell walls of mature stems and seedlings under salinity conditions, which probably contributes to the enhanced stem mechanical strength and improved salt tolerance in CYP75B4-OE plants. Our findings reveal a novel function of CYP75B4 in rice salt tolerance and lodging resistance by inducing tricin accumulation and lignin deposition in cell walls.

Dataset Information

OsCIPK9 Interacts with OsSOS3 and Affects Salt-Related Transport to Improve Salt Tolerance.

Publications

OsCIPK9 Interacts with OsSOS3 and Affects Salt-Related Transport to Improve Salt Tolerance.

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