Unknown

Dataset Information

0

Anti-Melanogenic Effects of Lilium lancifolium Root Extract via Downregulation of PKA/CREB and MAPK/CREB Signaling Pathways in B16F10 Cells.


ABSTRACT: Hyperpigmentation disorders causing emotional distress require the topical use of depigmenting agents of natural origin. In this study, the anti-melanogenic effects of the Lilium lancifolium root extract (LRE) were investigated in B16F10 cells. Consequently, a non-cytotoxic concentration of the extract reduced intracellular melanin content and tyrosinase activity in a dose-dependent manner, correlating with the diminished expression of core melanogenic enzymes within cells. LRE treatment also inhibited cyclic adenosine monophosphate (cAMP) response element-binding protein (CREB)/microphthalmia-associated transcription factor signaling, which regulates the expression of tyrosinase-related genes. Upon examining these findings from a molecular mechanism perspective, LRE treatment suppressed the phosphorylation of protein kinase A (PKA), p38, and extracellular signal-related kinase (ERK), which are upstream regulators of CREB. In addition, L-phenylalanine and regaloside A, specifically identified within the LRE using liquid chromatography-mass spectrometry, exhibited inhibitory effects on melanin production. Collectively, these results imply that LRE potentially suppresses cAMP-mediated melanogenesis by downregulating PKA/CREB and mitogen-activated protein kinase (MAPK)/CREB signaling pathways. Therefore, it can be employed as a novel therapeutic ingredient of natural origin to ameliorate hyperpigmentation disorders.

SUBMITTER: Park S 

PROVIDER: S-EPMC10648933 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature

altmetric image

Publications

Anti-Melanogenic Effects of <i>Lilium lancifolium</i> Root Extract via Downregulation of PKA/CREB and MAPK/CREB Signaling Pathways in B16F10 Cells.

Park Seokmuk S   Han Nayeon N   Lee Jungmin J   Lee Jae-Nam JN   An Sungkwan S   Bae Seunghee S  

Plants (Basel, Switzerland) 20231024 21


Hyperpigmentation disorders causing emotional distress require the topical use of depigmenting agents of natural origin. In this study, the anti-melanogenic effects of the <i>Lilium lancifolium</i> root extract (LRE) were investigated in B16F10 cells. Consequently, a non-cytotoxic concentration of the extract reduced intracellular melanin content and tyrosinase activity in a dose-dependent manner, correlating with the diminished expression of core melanogenic enzymes within cells. LRE treatment  ...[more]

Similar Datasets

| S-EPMC5855631 | biostudies-literature
| S-EPMC8068260 | biostudies-literature
| S-EPMC11677928 | biostudies-literature
| S-EPMC9737552 | biostudies-literature
| PRJNA428123 | ENA
| PRJNA419602 | ENA
| PRJNA418049 | ENA
| S-EPMC9645529 | biostudies-literature
| S-EPMC9504415 | biostudies-literature
| S-EPMC6778149 | biostudies-literature