Development of an efficient protein expression system in the thermophilic fungus Myceliophthora thermophila.

ABSTRACT:

Background

Thermophilic fungus Myceliophthora thermophila has been widely used in industrial applications due to its ability to produce various enzymes. However, the lack of an efficient protein expression system has limited its biotechnological applications.

Results

In this study, using a laccase gene reporting system, we developed an efficient protein expression system in M. thermophila through the selection of strong constitutive promoters, 5'UTRs and signal peptides. The expression of the laccase was confirmed by enzyme activity assays. The results showed that the Mtpdc promoter (Ppdc) was able to drive high-level expression of the target protein in M. thermophila. Manipulation of the 5'UTR also has significant effects on protein expression and secretion. The best 5'UTR

SUBMITTER: Li J 

PROVIDER: S-EPMC10652509 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature