NaNuTrap: a technique for in vivo cell nucleus labelling using nanobodies.

ABSTRACT: In vivo cell labelling is challenging in fast developmental processes because many cell types differentiate more quickly than the maturation time of fluorescent proteins, making visualization of these tissues impossible with standard techniques. Here, we present a nanobody-based method, Nanobody Nuclear Trap (NaNuTrap), which works with the existing Gal4/UAS system in Drosophila and allows for early in vivo cell nuclei labelling independently of the maturation time of the fluorescent protein. This restores the utility of fluorescent proteins that have longer maturation times, such as those used in two-photon imaging, for live imaging of fast or very early developmental processes. We also present a more general application of this system, whereby NaNuTrap can convert cytoplasmic GFP express

SUBMITTER: Akos Z 

PROVIDER: S-EPMC10656463 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature