A single-cell transcriptomic landscape of mouse testicular aging.

ABSTRACT:

Introduction

Advanced paternal age of reproduction is an increasing trend, especially in developed countries and areas. This trend results in elevated risks of adverse reproductive outcomes such as reduced fertility rates, increased pregnancy loss, and poor childhood health. Yet, a systematic profiling of aging-associated molecular and cellular alterations in testicular tissue is still missing.

Objectives

We aimed to dissect aging-associated molecular characteristics in testes of mice.

Methods

Single-cell transcriptomic sequencing and analysis were conducted in testes of young (2 months old) and old mice (24 months old). Immunofluorescences and immunochemistry were used to characterize aging-associated phenotypes and verify single cell sequence results.

Results

SUBMITTER: Zhang W 

PROVIDER: S-EPMC10658307 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature