Dengue virus exploits the host tRNA epitranscriptome to promote viral replication.

ABSTRACT: The 40-50 RNA modifications of the epitranscriptome regulate posttranscriptional gene expression. Here we show that flaviviruses hijack the host tRNA epitranscriptome to promote expression of pro-viral proteins, with tRNA-modifying ALKBH1 acting as a host restriction factor in dengue virus infection. Early in the infection of human Huh-7 cells, ALKBH1 and its tRNA products 5-formylcytidine (f⁵C) and 2'-O-methyl-5-formylcytidine (f⁵Cm) were reduced. ALKBH1 knockdown mimicked viral infection, but caused increased viral NS3 protein levels during infection, while ALKBH1 overexpression reduced NS3 levels and viral replication, and increased f⁵C and f⁵Cm. Viral NS5, but not host FTSJ1, increased f⁵Cm levels late in infection. Consiste

SUBMITTER: Chan C 

PROVIDER: S-EPMC10659268 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature