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Long-term live cell imaging during differentiation of human iPSC-derived neurons.

ABSTRACT: Live-cell imaging is crucial to appreciate the dynamics and the complexity of cellular interaction processes. However, live-cell imaging of human neurons is challenging due to neuronal sensitivity. Here, we describe a long-term live-cell imaging protocol for neurons derived from human induced pluripotent stem cells. By using an IncuCyte live-cell imaging system, we have obtained information on neuronal dynamics during the different stages of neurogenesis. The protocol has also been developed to monitor the dynamics of the neuronal intracellular organelles. For complete details on the use and execution of this protocol, please refer to Wang et al.¹.

SUBMITTER: Wang J

PROVIDER: S-EPMC10665918 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature

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Long-term live cell imaging during differentiation of human iPSC-derived neurons.

Wang Jingqi J Gleeson Paul A PA Fourriere Lou L

STAR protocols 20231107 4

Live-cell imaging is crucial to appreciate the dynamics and the complexity of cellular interaction processes. However, live-cell imaging of human neurons is challenging due to neuronal sensitivity. Here, we describe a long-term live-cell imaging protocol for neurons derived from human induced pluripotent stem cells. By using an IncuCyte live-cell imaging system, we have obtained information on neuronal dynamics during the different stages of neurogenesis. The protocol has also been developed to ...[more]

PMID: 37938977

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