Anti-complement factor H (CFH) autoantibodies could delay pristane-induced lupus nephritis.

ABSTRACT:

Purpose

Anti-complement factor H (CFH) autoantibodies could be detected in lupus and its significance remained to be elucidated. Herein, we aimed to explore the roles of anti-CFH autoantibodies based on pristane-induced lupus mice.

Methods

Twenty-four female Balb/c mice were randomly divided into four groups, with one group injected with pristane (pristane group), one group with pristane and then human CFH (hCFH) (pristane-CFH group) 3 times, and the other two as vertical controls, PBS group and PBS-CFH group. Histopathological analysis was performed six months after pristane administration. Levels of hCFH, anti-CFH autoantibodies and anti-dsDNA antibody were detected. Murine IgG (mIgG) were purified and cross-reactivity, epitopes, subclasses and functional analysis were fur

SUBMITTER: Li LL 

PROVIDER: S-EPMC10667379 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature