Depolymerisation of Kraft Lignin by Tailor-Made Alkaliphilic Fungal Laccases.

ABSTRACT: Lignins released in the black liquors of kraft pulp mills are an underutilised source of aromatics. Due to their phenol oxidase activity, laccases from ligninolytic fungi are suitable biocatalysts to depolymerise kraft lignins, which are characterised by their elevated phenolic content. However, the alkaline conditions necessary to solubilise kraft lignins make it difficult to use fungal laccases whose activity is inherently acidic. We recently developed through enzyme-directed evolution high-redox potential laccases active and stable at pH 10. Here, the ability of these tailor-made alkaliphilic fungal laccases to oxidise, demethylate, and depolymerise eucalyptus kraft lignin at pH 10 is evidenced by the increment in the content of phenolic hydroxyl and carbonyl groups, the methanol releas

SUBMITTER: Rodriguez-Escribano D 

PROVIDER: S-EPMC10675753 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature