Characterization of human placenta-derived exosome (pExo) as a potential osteoarthritis disease modifying therapeutic.

ABSTRACT:

Objective

Human placenta-derived exosomes (pExo) were generated, characterized, and evaluated as a therapeutic candidate for the treatment of osteoarthritis (OA).

Methods

pExo was generated from full-term human placenta tissues by sequential centrifugation, purification, and sterile filtration. Upon analysis of particle size, cytokine composition, and exosome marker expression, pExo was further tested in cell-based assays to examine its effects on human chondrocytes. In vivo therapeutic efficacies were evaluated in a medial meniscal tear/medial collateral ligament tear (MCLT + MMT) rat model, in which animals received pExo injections intraarticularly and weight bearing tests during in-life stage while histopathology and immunohistochemistry were performed as terminal endpoin

SUBMITTER: Huang C 

PROVIDER: S-EPMC10683254 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature