Chikungunya virus nonstructural protein 1 is a versatile RNA capping and decapping enzyme.

ABSTRACT: Chikungunya virus (CHIKV) nonstructural protein 1 (nsP1) contains both the N7-guanine methyltransferase and guanylyltransferase activities and catalyzes the 5' end cap formation of viral RNAs. To further understand its catalytic activity and role in virus-host interaction, we demonstrate that purified recombinant CHIKV nsP1 can reverse the guanylyl transfer reaction and remove the m⁷GMP from a variety of capped RNA substrates including host mRNAs. We then provide the structural basis of this function with a high-resolution cryo-EM structure of nsP1 in complex with the unconventional cap-1 substrate RNA m⁷GpppA_mU. We show that the 5'ppRNA species generated by decapping can trigger retinoic acid-inducible gene I-mediated interferon response. We further demons

SUBMITTER: Law MCY 

PROVIDER: S-EPMC10687048 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature