Ontology highlight
ABSTRACT:
SUBMITTER: Law MCY
PROVIDER: S-EPMC10687048 | biostudies-literature | 2023 Oct
REPOSITORIES: biostudies-literature
Law Michelle Cheok Yien MCY Zhang Kuo K Tan Yaw Bia YB Nguyen Trinh Mai TM Luo Dahai D
The Journal of biological chemistry 20231031 12
Chikungunya virus (CHIKV) nonstructural protein 1 (nsP1) contains both the N7-guanine methyltransferase and guanylyltransferase activities and catalyzes the 5' end cap formation of viral RNAs. To further understand its catalytic activity and role in virus-host interaction, we demonstrate that purified recombinant CHIKV nsP1 can reverse the guanylyl transfer reaction and remove the m<sup>7</sup>GMP from a variety of capped RNA substrates including host mRNAs. We then provide the structural basis ...[more]