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Versatile strategy using vaccinia virus-capping enzyme to synthesize functional 5' cap-modified mRNAs.

ABSTRACT: The potential of synthetic mRNA as a genetic carrier has increased its application in scientific fields. Because the 5' cap regulates the stability and translational activity of mRNAs, there are concerted efforts to search for and synthesize chemically-modified 5' caps that improve the functionality of mRNA. Here, we report an easy and efficient method to synthesize functional mRNAs by modifying multiple 5' cap analogs using a vaccinia virus-capping enzyme. We show that this enzyme can introduce a variety of GTP analogs to the 5' end of RNA to generate 5' cap-modified mRNAs that exhibit different translation levels. Notably, some of these modified mRNAs improve translation efficiency and can be conjugated to chemical structures, further increasing their functionality. Our versatile method to generate 5' cap-modified mRNAs will provide useful tools for RNA therapeutics and biological research.

SUBMITTER: Ohno H

PROVIDER: S-EPMC10085709 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature

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Versatile strategy using vaccinia virus-capping enzyme to synthesize functional 5' cap-modified mRNAs.

Ohno Hirohisa H Akamine Sae S Mochizuki Megumi M Hayashi Karin K Akichika Shinichiro S Suzuki Tsutomu T Saito Hirohide H

Nucleic acids research 20230401 6

The potential of synthetic mRNA as a genetic carrier has increased its application in scientific fields. Because the 5' cap regulates the stability and translational activity of mRNAs, there are concerted efforts to search for and synthesize chemically-modified 5' caps that improve the functionality of mRNA. Here, we report an easy and efficient method to synthesize functional mRNAs by modifying multiple 5' cap analogs using a vaccinia virus-capping enzyme. We show that this enzyme can introduce ...[more]

PMID: 36731515

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A metazoan/plant-like capping enzyme and cap modified nucleotides in the unicellular eukaryote Trichomonas vaginalis.

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Identification of re-capping substrates for the cytoplasmic capping enzyme complex

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2012-12-20 | E-GEOD-36729 | biostudies-arrayexpress

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Identification of re-capping substrates for the cytoplasmic capping enzyme complex

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2012-12-20 | GSE36729 | GEO

Novel Bifunctional Acylase from Actinoplanes utahensis: A Versatile Enzyme to Synthesize Antimicrobial Compounds and Use in Quorum Quenching Processes.

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