Benchmarking of virome metagenomic analysis approaches using a large, 60+ members, viral synthetic community.

ABSTRACT:

Importance

We report here efforts to benchmark performance of two widespread approaches for virome analysis, which target either virion-associated nucleic acids (VANA) or highly purified double-stranded RNAs (dsRNAs). This was achieved using synthetic communities of varying complexity levels, up to a highly complex community of 72 viral agents (115 viral molecules) comprising isolates from 21 families and 61 genera of plant viruses. The results obtained confirm that the dsRNA-based approach provides a more complete representation of the RNA virome, in particular, for high complexity ones. However, for viromes of low to medium complexity, VANA appears a reasonable alternative and would be the preferred choice if analysis of DNA viruses is of importance. Several parameters impacting

SUBMITTER: Schonegger D 

PROVIDER: S-EPMC10688312 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature