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Microflow injection analysis based on modular 3D platforms and colorimetric detection for Fe(III) monitoring in a wide concentration range.

ABSTRACT: A modular microflow injection analysis (microFIA) system for the determination of Fe(III) in a bioleaching reactor has been designed, developed and validated. The different modules of the analyzer (mixer, diluter, disperser and detector) were 3D-printed. Fe(III) quantification is due by measuring the color intensity of the chelate formed between Fe(III) and salicylic acid at 525 nm. The device has been designed to dilute, disperse and detect high Fe(III) concentrations in the form of an inexpensive multi-step photometric flow cell that uses an light-emitting diode (LED) as a light source and an light-dependent resistor (LDR) as a light intensity detector. This microFIA system has been shown to be suitable for automatic and continuous determination of Fe(III) in the operation of a bioreactor for the oxidation of Fe(II). The device has a good repeatability (less than 5% of coefficient of variation in the whole range of concentrations) and accuracy of around 100%. The analyzer features an exceptional wide linear range, between 25 and 6000 mg·L^-1. The device was successfully applied to the determination of Fe(III) in real samples. The obtained results proved that the method is applicable for accurate, precise, rapid, and low-cost colorimetric analysis and didn't show significant differences with a conventional UV-Vis method.

SUBMITTER: Ricart D

PROVIDER: S-EPMC10693521 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature

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Microflow injection analysis based on modular 3D platforms and colorimetric detection for Fe(III) monitoring in a wide concentration range.

Ricart David D Dorado Antonio David AD Lao-Luque Conxita C Baeza Mireia M

Mikrochimica acta 20231202 1

A modular microflow injection analysis (microFIA) system for the determination of Fe(III) in a bioleaching reactor has been designed, developed and validated. The different modules of the analyzer (mixer, diluter, disperser and detector) were 3D-printed. Fe(III) quantification is due by measuring the color intensity of the chelate formed between Fe(III) and salicylic acid at 525 nm. The device has been designed to dilute, disperse and detect high Fe(III) concentrations in the form of an inexpens ...[more]

PMID: 38041754

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Project description:Whole-genome DNA microarray analysis of Geobacter sulfurreducens cells grown on Fe(III)-oxide or Mn(IV)-oxide versus cells grown on soluble Fe(III) citrate indicated that there were significant differences in transcription patterns during growth on the insoluble metal oxides compared to growth on soluble Fe(III). Many of the genes that appeared to be up-regulated during growth on the metal hydroxides were involved in electron transport. The most highly up-regulated genes for both conditions were omcS and omcT, which encode co-transcribed c-type cytochromes exposed on the outer surface of the cell that are known to be required for Fe(III) and Mn(IV)-oxide reduction. Other electron transport genes that were up-regulated on both insoluble metals included the gene coding for the outer membrane c-type cytochrome, OmcG, genes for the outer membrane proteins, OmpB and OmpC, and the gene that codes for the structural protein of electrically conductive pili, PilA. Genes that were up-regulated in cells grown on Fe(III)-oxide but not Mn(IV)-oxide, included outer membrane c-type cytochromes including OmcE, a putative DMSO reductase protein, and proteins from the cytochrome bc1 complex. Electron transport genes that were only up-regulated in Mn(IV)-oxide grown cells included the genes that code for the outer membrane c-type cytochromes, OmcZ and OmcB, the periplasmic c-type cytochrome, MacA, and fumarate reductase. Genetic studies indicated that the c-type cytochrome proteins, PpcH, OmcJ, OmcM, OmcV, MacA, OmcF, OmcI, and OmcQ, and the iron sulfur subunit of the cytochrome b/b6 complex, QcrA, are important for reduction of insoluble Fe(III)-oxides but do not appear to be important for Mn(IV) reduction. These results demonstrate that the physiology of Fe(III) reducing bacteria differ significantly during growth on insoluble electron and soluble electron acceptors and emphasizes the importance of c-type cytochromes in extracellular electron transfer in G. sulfurreducens. Geobacter sulfurreducens cells were grown with acetate (5 mM) provided as the electron donor and either Fe(III) oxide or Fe(III) citrate provided as the electron acceptor. Cells were harvested at mid-log and total RNA was extracted. Total RNA (0.5 μg) was amplified using the MessageAmp II-Bacteria Kit (Ambion, Foster City, CA) according to the manufacturers instructions. Ten micrograms of amplified RNA (aRNA) was chemically labeled with Cy3 (for the control or soluble electron acceptor condition) or Cy5 (for the experimental or insoluble electron acceptor condition) dye using the MicroMax ASAP RNA Labeling Kit (Perkin Elmer, Wellesley, MA) according to the manufacturer’s instructions.

Project description:Whole-genome DNA microarray analysis of Geobacter sulfurreducens cells grown on Fe(III)-oxide or Mn(IV)-oxide versus cells grown on soluble Fe(III) citrate indicated that there were significant differences in transcription patterns during growth on the insoluble metal oxides compared to growth on soluble Fe(III). Many of the genes that appeared to be up-regulated during growth on the metal hydroxides were involved in electron transport. The most highly up-regulated genes for both conditions were omcS and omcT, which encode co-transcribed c-type cytochromes exposed on the outer surface of the cell that are known to be required for Fe(III) and Mn(IV)-oxide reduction. Other electron transport genes that were up-regulated on both insoluble metals included the gene coding for the outer membrane c-type cytochrome, OmcG, genes for the outer membrane proteins, OmpB and OmpC, and the gene that codes for the structural protein of electrically conductive pili, PilA. Genes that were up-regulated in cells grown on Fe(III)-oxide but not Mn(IV)-oxide, included outer membrane c-type cytochromes including OmcE, a putative DMSO reductase protein, and proteins from the cytochrome bc1 complex. Electron transport genes that were only up-regulated in Mn(IV)-oxide grown cells included the genes that code for the outer membrane c-type cytochromes, OmcZ and OmcB, the periplasmic c-type cytochrome, MacA, and fumarate reductase. Genetic studies indicated that the c-type cytochrome proteins, PpcH, OmcJ, OmcM, OmcV, MacA, OmcF, OmcI, and OmcQ, and the iron sulfur subunit of the cytochrome b/b6 complex, QcrA, are important for reduction of insoluble Fe(III)-oxides but do not appear to be important for Mn(IV) reduction. These results demonstrate that the physiology of Fe(III) reducing bacteria differ significantly during growth on insoluble electron and soluble electron acceptors and emphasizes the importance of c-type cytochromes in extracellular electron transfer in G. sulfurreducens. Geobacter sulfurreducens cells were grown with acetate (5 mM) provided as the electron donor and either Fe(III) oxide or Fe(III) citrate provided as the electron acceptor. Cells were harvested at mid-log and total RNA was extracted. Total RNA (0.5 M-NM-<g) was amplified using the MessageAmp II-Bacteria Kit (Ambion, Foster City, CA) according to the manufacturers instructions. Ten micrograms of amplified RNA (aRNA) was chemically labeled with Cy3 (for the control or soluble electron acceptor condition) or Cy5 (for the experimental or insoluble electron acceptor condition) dye using the MicroMax ASAP RNA Labeling Kit (Perkin Elmer, Wellesley, MA) according to the manufacturerM-bM-^@M-^Ys instructions. RNA samples from three biological replicates were hybridized in duplicate on 12K Combimatrix antisense-detecting arrays. The experimental condition (DL1 grown with Fe(III) oxide as acceptor) was labeled with cy5, the control condition (DL1 grown with Fe(III) citrate as acceptor) was labeled with cy3

Dataset Information

Microflow injection analysis based on modular 3D platforms and colorimetric detection for Fe(III) monitoring in a wide concentration range.

Publications

Microflow injection analysis based on modular 3D platforms and colorimetric detection for Fe(III) monitoring in a wide concentration range.

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