Nicotinamide mononucleotide impacts HIV-1 infection by modulating immune activation in T lymphocytes and humanized mice.

ABSTRACT:

Background

HIV-1-associated immune activation drives CD4⁺ T cell depletion and the development of acquired immunodeficiency syndrome. We aimed to determine the role of nicotinamide mononucleotide (NMN), the direct precursor of nicotinamide adenine dinucleotide (NAD) co-enzyme, in CD4⁺ T cell modulation during HIV-1 infection.

Methods

We examined HIV-1 integrated DNA or transcribed RNA, intracellular p24 protein, and T cell activation markers in CD4⁺ T cells including in vitro HIV-1-infected cells, reactivated patient-derived cells, and in HIV-1-infected humanized mice, under NMN treatment. RNA-seq and CyTOF analyses were used for investigating the effect of NMN on CD4⁺ T cells.

Findings

We found that NMN increased the intrac

SUBMITTER: Mo Y 

PROVIDER: S-EPMC10694053 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature