Correlation between membrane proteins and sizes of extracellular vesicles and particles: A potential signature for cancer diagnosis.

ABSTRACT: Extracellular vesicles and particles (EVPs) are recognized as ideal liquid biopsy tools for cancer detection, and membrane proteins are commonly used EVP biomarkers. However, bulk analysis of EVP membrane protein biomarkers typically fails to meet the clinical requirement for diagnostic accuracy. We investigated the correlation between the membrane protein expression level, the binding kinetics to aptamers and the sizes of EVPs with interferometric plasmonic microscopy (iPM), and demonstrated the implementation of the correlative signature to determine cancer types. Using EVPs collected from both cell model and clinical plasma samples with liver, lung, breast, or prostate cancer, we found that the selective set of membrane protein expression levels of five protein markers and their binding

SUBMITTER: Zhai C 

PROVIDER: S-EPMC10696525 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature