A study implementing real-time PCR to identify Strongyloides species of third-stage larvae in human stool samples from Southern Vietnam.

ABSTRACT:

Background

Strongyloidiasis, a neglected disease caused by intestinal nematodes of the genus, is endemic to tropical and subtropical areas such as Vietnam. Morphological methods only identify the genus, while DNA-molecular techniques are susceptible in Strongyloides spp. detection. The study aims to determine the prevalence of dominant Strongyloides species among the population in Duc Hoa district, Long An, Vietnam.

Methods

A cross-sectional study used 1190 stool specimens collected from July 2017 to November 2018. All samples were transported within 2 h, stored at 2-8°C, and processed within 48 h for microscopy smear and culture at the Laboratory of Medical Parasitology, Pham Ngoc Thach University of Medicine (PNT). Then all positive samples with the above 2 m

SUBMITTER: Vinh LD 

PROVIDER: S-EPMC10699675 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature