Spatial architecture of high-grade glioma reveals tumor heterogeneity within distinct domains.

ABSTRACT:

Background

High-grade gliomas (HGGs) are aggressive primary brain cancers with poor response to standard regimens, driven by immense heterogeneity. In isocitrate dehydrogenase (IDH) wild-type HGG (glioblastoma, GBM), increased intratumoral heterogeneity is associated with more aggressive disease.

Methods

Spatial technologies can dissect complex heterogeneity within the tumor ecosystem by preserving cellular organization in situ. We employed GeoMx digital spatial profiling, CosMx spatial molecular imaging, Xenium in situ mapping and Visium spatial gene expression in experimental and validation patient cohorts to interrogate the transcriptional landscape in HGG.

Results

Here, we construct a high-resolution molecular map of heterogeneity in GBM and <

SUBMITTER: Moffet JJD 

PROVIDER: S-EPMC10699851 | biostudies-literature | 2023 Jan-Dec

REPOSITORIES: biostudies-literature