Single-cell histone chaperones patterns guide intercellular communication of tumor microenvironment that contribute to breast cancer metastases.

ABSTRACT:

Background

Histone chaperones (HCs) are crucial for governing genome stability and gene expression in multiple cancers. However, the functioning of HCs in the tumor microenvironment (TME) is still not clearly understood.

Methods

Self-tested single-cell RNA-seq data derived from 6 breast cancer (BC) patients with brain and liver metastases were reanalyzed by nonnegative matrix factorization (NMF) algorithm for 36 HCs. TME subclusters were observed with BC and immunotherapy public cohorts to assess their prognosis and immune response. The biological effect of HSPA8, one of the HCs, was verified by transwell assay and wound-healing assays.

Results

Cells including fibroblasts, macrophages, B cells, and T cells, were classified into various subclusters based on marker gene

SUBMITTER: Xie J 

PROVIDER: S-EPMC10702093 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature