Cheaper, faster, simpler trypsin digestion for high-throughput targeted protein quantification.

ABSTRACT:

Introduction

LC-MS-based methods for protein quantification have a stigma of being relatively expensive and low-throughput. This is partly due to the cost and speed of trypsin digestion, which has primarily focused on advancements in research-based biomarker discovery applications that rely on protein/peptide identifications rather than clinical biomarker quantification. However, there is a need for simple, fast, and reproducibly efficient surrogate peptide recovery in clinical biomarker quantification.

Methods

Multiple methodologies were evaluated to enhance tryptic digestion for the analysis of thyroglobulin, a prototypical serum protein biomarker. The main criteria for assessment were the yield and speed of formation of surrogate peptides. Various factors such as differen

SUBMITTER: Shuford CM 

PROVIDER: S-EPMC10716584 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature