Activated protein C inhibits mesothelial-to-mesenchymal transition in experimental peritoneal fibrosis.

ABSTRACT:

Background

In addition to its anticoagulant function in downregulating thrombin generation, activated protein C (APC) evokes pleiotropic cytoprotective signaling activities when it binds to endothelial protein C receptor (EPCR) to activate protease-activated receptor 1 (PAR1) in endothelial cells.

Objectives

To investigate the protective effect of APC in a chlorhexidine gluconate (CG)-induced peritoneal fibrosis model.

Methods

Peritoneal fibrosis was induced in wild-type as well as EPCR- and PAR1-deficient mice via daily injection of CG (0.2 mL of 0.1% CG in 15% ethanol and 85% saline) for 21 days with or without concomitant injection of recombinant human APC derivatives (50 μg/kg of bodyweight). The expression of proinflammatory cytokines and profibrotic markers as w

SUBMITTER: Giri H 

PROVIDER: S-EPMC10726528 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature