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Dataset describing the influence of culture conditions on the bioreduction of organic acids to alcohols by Thermoanaerobacter pseudethanolicus.

ABSTRACT: The dataset describes the influence of culture conditions on the bioreduction of organic acids by Thermoanaerobacter pseudethanolicus as reported in [1]. The data shows that during glucose fermentation of Thermoanaerobacter pseudethanolicus the reducing equivalents are not only converted to ethanol and hydrogen but also, in the presence of carboxylic acids (C2-C6), to its corresponding alcohol. To maximize the alcohol production produced from their carboxylic acid, several experiments were performed to investigate the effect of various environmental factors (initial glucose concentration, pH, liquid-gas phase ratio, and inhibitory effects of alcohols) on growth. A kinetic experiment of glucose in the absence and presence of selected fatty acids are also presented as are data on selected enzyme activities related to alcohols and aldehydes and a time course study of the reduction of ¹³C1 labeled butyrate using glucose as a carbon source.

SUBMITTER: Orlygsson J

PROVIDER: S-EPMC10751834 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature

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Dataset describing the influence of culture conditions on the bioreduction of organic acids to alcohols by Thermoanaerobacter pseudethanolicus.

Orlygsson Johann J Scully Sean Michael SM

Data in brief 20231215

The dataset describes the influence of culture conditions on the bioreduction of organic acids by Thermoanaerobacter pseudethanolicus as reported in [1]. The data shows that during glucose fermentation of Thermoanaerobacter pseudethanolicus the reducing equivalents are not only converted to ethanol and hydrogen but also, in the presence of carboxylic acids (C2-C6), to its corresponding alcohol. To maximize the alcohol production produced from their carboxylic acid, several experime ...[more]

PMID: 38152501

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Project description:BackgroundChemical and physical pretreatment of lignocellulosic biomass improves substrate reactivity for increased microbial biofuel production, but also restricts growth via the release of furan aldehydes, such as furfural and 5-hydroxymethylfurfural (5-HMF). The physiological effects of these inhibitors on thermophilic, fermentative bacteria are important to understand; especially as cellulolytic strains are being developed for consolidated bioprocessing (CBP) of lignocellulosic feedstocks. Identifying mechanisms for detoxification of aldehydes in naturally resistant strains, such as Thermoanaerobacter spp., may also enable improvements in candidate CBP microorganisms.ResultsThermoanaerobacter pseudethanolicus 39E, an anaerobic, saccharolytic thermophile, was found to grow readily in the presence of 30 mM furfural and 20 mM 5-HMF and reduce these aldehydes to their respective alcohols in situ. The proteomes of T. pseudethanolicus 39E grown in the presence or absence of 15 mM furfural were compared to identify upregulated enzymes potentially responsible for the observed reduction. A total of 225 proteins were differentially regulated in response to the 15 mM furfural treatment with 152 upregulated versus 73 downregulated. Only 87 proteins exhibited a twofold or greater change in abundance in either direction. Of these, 54 were upregulated in the presence of furfural and 33 were downregulated. Two oxidoreductases were upregulated at least twofold by furfural and were targeted for further investigation. Teth39_1597 encodes a predicted butanol dehydrogenase (BdhA) and Teth39_1598, a predicted aldo/keto reductase (AKR). Both genes were cloned from T. pseudethanolicus 39E, with the respective enzymes overexpressed in E. coli and specific activities determined against a variety of aldehydes. Overexpressed BdhA showed significant activity with all aldehydes tested, including furfural and 5-HMF, using NADPH as the cofactor. Cell extracts with AKR also showed activity with NADPH, but only with four-carbon butyraldehyde and isobutyraldehyde.ConclusionsT. pseudethanolicus 39E displays intrinsic tolerance to the common pretreatment inhibitors furfural and 5-HMF. Multidimensional proteomic analysis was used as an effective tool to identify putative mechanisms for detoxification of furfural and 5-HMF. T. pseudethanolicus was found to upregulate an NADPH-dependent alcohol dehydrogenase 6.8-fold in response to furfural. In vitro enzyme assays confirmed the reduction of furfural and 5-HMF to their respective alcohols.

Dataset Information

Dataset describing the influence of culture conditions on the bioreduction of organic acids to alcohols by Thermoanaerobacter pseudethanolicus.

Publications

Dataset describing the influence of culture conditions on the bioreduction of organic acids to alcohols by <i>Thermoanaerobacter pseudethanolicus</i>.

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