A Perspective on Developing Modeling and Image Analysis Tools to Investigate Mechanosensing Proteins.

ABSTRACT: The shift of funding organizations to prioritize interdisciplinary work points to the need for workflow models that better accommodate interdisciplinary studies. Most scientists are trained in a specific field and are often unaware of the kind of insights that other disciplines could contribute to solving various problems. In this paper, we present a perspective on how we developed an experimental pipeline between a microscopy and image analysis/bioengineering lab. Specifically, we connected microscopy observations about a putative mechanosensing protein, obscurin, to image analysis techniques that quantify cell changes. While the individual methods used are well established (fluorescence microscopy; ImageJ WEKA and mTrack2 programs; MATLAB), there are no existing best practices for how to

SUBMITTER: Ouderkirk S 

PROVIDER: S-EPMC10755202 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature