Enzymatic properties and inhibition tolerance analysis of key enzymes in β-phenylethanol anabolic pathway of Saccharomyces cerevisiae HJ.

ABSTRACT: Huangjiu is known for its unique aroma, primarily attributed to its high concentration of β-phenylethanol (ranging from 40 to 130 mg/L). Phenylalanine aminotransferase Aro9p and phenylpyruvate decarboxylase Aro10p are key enzymes in the β-phenylethanol synthetic pathway of Saccharomyces cerevisiae HJ. This study examined the enzymatic properties of these two enzymes derived from S. cerevisiae HJ and S288C. After substrate docking, Aro9p^HJ (-24.05 kJ/mol) and Aro10p^HJ (-14.33 kJ/mol) exhibited lower binding free energies compared to Aro9p^S288C (-21.93 kJ/mol) and Aro10p^S288C (-12.84 kJ/mol). ARO9 and ARO10 genes were heterologously expressed in E. coli BL21. Aro9p, which was purified via affinity chromatog

SUBMITTER: Yang Q 

PROVIDER: S-EPMC10755794 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature