Molecular Detectıon of Canine Leishmaniasis in Northern Anatolia, Turkiye.

ABSTRACT:

Background

Canin leishmaniasis (CanL), mostly caused by Leishmania infantum, is one of the most important vector-borne diseases in dogs in the Mediterranean region. In this study, we aimed to determine the disease profile in this region by firstly making microscopic and then molecular analyzes in the samples taken from the dogs.

Methods

Overall, 112 whole blood samples taken from dogs for clinical applications by a veterinarian in Cankırı between December 2021 and November 2022 were used. After blood collection, both thin and thick drop blood smear preparations were prepared and evaluated for Giemsa staining. L. infantum was investigated by Real time-PCR (RT-PCR) method from all blood samples. Sequence analysis and phylogenetic tree study were performed on posi

SUBMITTER: Yucesan B 

PROVIDER: S-EPMC10758073 | biostudies-literature | 2023 Oct-Dec

REPOSITORIES: biostudies-literature