Doxycycline for transgene control disrupts gut microbiome diversity without compromising acute neuroinflammatory response.

ABSTRACT: The tetracycline transactivator (tTA) system provides controllable transgene expression through oral administration of the broad-spectrum antibiotic doxycycline. Antibiotic treatment for transgene control in mouse models of disease might have undesirable systemic effects resulting from changes in the gut microbiome. Here we assessed the impact of doxycycline on gut microbiome diversity in a tTA-controlled model of Alzheimer's disease and then examined neuroimmune effects of these microbiome alterations following acute LPS challenge. We show that doxycycline decreased microbiome diversity in both transgenic and wild-type mice and that these changes persisted long after drug withdrawal. Despite the change in microbiome composition, doxycycline treatment had minimal effect on basal transcript

SUBMITTER: Koller EJ 

PROVIDER: S-EPMC10765643 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature