MosGILT controls innate immunity and germ cell development in Anopheles gambiae.

ABSTRACT: Gene-edited mosquitoes lacking a gamma-interferon-inducible lysosomal thiol reductase-like protein, namely (mosGILT^null) have lower Plasmodium infection, which is linked to impaired ovarian development and immune activation. The transcriptome of mosGILT^null Anopheles gambiae was therefore compared to wild type (WT) mosquitoes by RNA-sequencing to delineate mosGILT-dependent pathways. Compared to WT mosquitoes, mosGILT^null A. gambiae demonstrated altered expression of genes related to oogenesis, 20-hydroxyecdysone synthesis, as well as immune-related genes. Serendipitously, the zero population growth gene, zpg, an essential regulator of germ cell development was found to be one of the most downregulated genes in mosGILT^null mosquitoes. These resul

SUBMITTER: Arora G 

PROVIDER: S-EPMC10775533 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature