Effects of adipose-derived mesenchymal stem cell conditioned medium on human tenocytes exposed to high glucose.

ABSTRACT:

Introduction

Diabetic tendinopathy is a common invalidating and challenging disease that may be treated using stem cells. However, the effects of adipose-derived mesenchymal stem cell conditioned medium (ASC-CM) in diabetic tendinopathy have never been explored.

Objectives

The present study evaluated the effects of ASC-CM on morphology, cell viability, structure, and scratch wound closure of human tenocytes (HTNC) exposed to high glucose (HG).

Design

Experimental study.

Methods

HTNC were exposed to HG (25 mM) for 7, 14 and 21 days with or without ASC-CM for the last 24 h. CM was collected from 4 × 10⁵ ASCs, centrifuged for 10 min at 200 g and sterilized with 0.22 μm syringe filter.

Results

At 7 days, HG-HTNC had decreased cell viability [72 ± 

SUBMITTER: Trotta MC 

PROVIDER: S-EPMC10775729 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature