Dithioethanol (DTE)-Conjugated Deoxyribose Cyclic Dinucleotide Prodrugs (DTE-dCDNs) as STING Agonist.

ABSTRACT: To improve the chemical regulation on the activity of cyclic dinucleotides (CDNs), we here designed a reduction-responsive dithioethanol (DTE)-based dCDN prodrug 9 (DTE-dCDN). Prodrug 9 improved the cell permeability with the intracellular levels peaking in 2 h in THP-1 cells. Under the reductive substance such as GSH or DTT, prodrug 9 could be quickly decomposed in 30 min to release the parent dCDN. In THP1-Lucia cells, prodrug 9 also retained a high bioactivity with the EC₅₀ of 0.96 μM, which was 51-, 43-, and 3-fold more than the 2',3'-cGAMP (EC₅₀ = 48.6 μM), the parent compound 3',3'-c-di-dAMP (EC₅₀ = 41.3 μM), and ADU-S100 (EC₅₀ = 2.9 μM). The high bioactivity of prodrug 9 was validated to be highly correlated with the activation of the STING signaling pathway. Furthermore, prodrug 9 could also improve the transcriptional expression levels of IFN-β, CXCL10, IL-6, and TNF-α in THP-1 cells. These results will be helpful to the development of chemically controllable CDN prodrugs with a high cellular permeability and potency.

SUBMITTER: Xie Z 

PROVIDER: S-EPMC10778758 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature