Overexpression of genes involved in fatty acid biosynthesis increases lipid content in the NaHCO3-tolerant Chlorella sp. JB6.
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ABSTRACT: Importance
Fatty acid (FA) contents can be altered in Chlorella JB6 in the presence of sodium bicarbonate (NaHCO3). Overexpression of the FA de novo synthesis genes inhibited the growth of JB6 cells and decreased their resistance to NaHCO3, but these transgenic JB6 strains could grow in a medium containing as high as 300 mM NaHCO3. In JB6, ectopic expression of the FA de novo synthesis genes increased the synthesis of very long-chain saturated FA (> 20C).
SUBMITTER: Shang H
PROVIDER: S-EPMC10783073 | biostudies-literature | 2024 Jan
REPOSITORIES: biostudies-literature
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Overexpression of genes involved in fatty acid biosynthesis increases lipid content in the NaHCO<sub>3</sub>-tolerant <i>Chlorella</i> sp. JB6.
Shang Hongna H Liu Songsong S Xu Chenghui C Liu Shenkui S Liu Hua H
Microbiology spectrum 20231204 1
<h4>Importance</h4>Fatty acid (FA) contents can be altered in Chlorella JB6 in the presence of sodium bicarbonate (NaHCO<sub>3</sub>). Overexpression of the FA <i>de novo</i> synthesis genes inhibited the growth of JB6 cells and decreased their resistance to NaHCO<sub>3</sub>, but these transgenic JB6 strains could grow in a medium containing as high as 300 mM NaHCO<sub>3</sub>. In JB6, ectopic expression of the FA <i>de novo</i> synthesis genes increased the synthesis of very long-chain saturat ...[more]
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