Overexpression of genes involved in fatty acid biosynthesis increases lipid content in the NaHCO₃-tolerant Chlorella sp. JB6.

ABSTRACT:

Importance

Fatty acid (FA) contents can be altered in Chlorella JB6 in the presence of sodium bicarbonate (NaHCO₃). Overexpression of the FA de novo synthesis genes inhibited the growth of JB6 cells and decreased their resistance to NaHCO₃, but these transgenic JB6 strains could grow in a medium containing as high as 300 mM NaHCO₃. In JB6, ectopic expression of the FA de novo synthesis genes increased the synthesis of very long-chain saturated FA (> 20C).

SUBMITTER: Shang H 

PROVIDER: S-EPMC10783073 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature